Abstract:

Colon cancer has been the second most common cause of death and accounts for almost 10% of cancer deaths. In particular, the biomaterial-based drug delivery system, with advantage of self-propelling and colon cancer targeting, could function as facilitator to achieve effective anti-cancer therapy. Here, after conjugation of folic acid (FA) with zein, we formulate a core-shell Zein-based nanoparticle (Zein NP), consisting of Doxorubicin (DOX)-encapsulated zein core and catalase (CAT)-loaded shell. By entrapping the Zein NP into arabinoxylan hydrogel, this delivery system can achieve gel-sol transition and facilitate DOX colon-target delivery. The physicochemical properties, self-propelling behavior, drug release profile of Zein NP were evaluated. The in-vitro cytotoxicity, biocompatibility and therapeutic efficacy of Zein NP were also examined by HeLa cell model and azoxy menthane-dextran sodium (AOM-DSS)-induced tumor animal models. Results showed that Zein NP exhibited spherical, well-defined and monodispersed morphology. It presented self-propelling motion due to the catalytic decomposition of hydrogen peroxide by CAT. The in vitro cumulative release curves showed that Zein NP kept stable in gastric and small intestine fluid, and colon-responsive release behavior. In vivo, Zein NP mostly released and accumulated in colon, exhibit great DOX targeting delivery efficiency. In brief, this study provides an ideal all-natural oral colon-target drug delivery system to treat colon cancer.

ALL INTERESTED ARE WELCOME